Raw Cow’s Milk Prevents the Development of Airway Inflammation in a Murine House Dust Mite-Induced Asthma Model

 

Abstract

Epidemiological studies show an inverse relation between raw cow’s milk consumption and the development of asthma. This protective effect seems to be abolished by milk processing. However, evidence for a causal relationship is lacking, and direct comparisons between raw and processed milk are hardly studied. Therefore, this study investigated the preventive capacity of raw and heated raw milk on the development of house dust mite (HDM)-induced allergic asthma in mice. Six- to seven-week-old male BALB/c mice were intranasally (i.n.) sensitized with 1 µg HDM or PBS on day 0, followed by an i.n. challenge with 10 µg HDM or PBS on days 7–11. In addition, mice were fed 0.5 mL raw cow’s milk, heated raw cow’s milk, or PBS three times a week throughout the study, starting 1 day before sensitization. On day 14, airway hyperresponsiveness (AHR) in response to increasing doses of methacholine was measured to assess lung function. Bronchoalveolar lavage fluid (BALF) and lungs were furthermore collected to study the extent of airway inflammation. Raw milk prevented both HDM-induced AHR and pulmonary eosinophilic inflammation, whereas heated raw milk did not. Both milk types suppressed the Th2-polarizing chemokine CCL17 in lung homogenates and reduced lung Th2 and Th17 cell frequency. IL-4 and IL-13 production after ex vivo restimulation of lung T cells with HDM was also reduced by both milk types. However, local IL-5 and IL-13 concentrations were only suppressed by raw milk. These findings support the asthma-protective capacity of raw cow’s milk and show the importance of reduced local type 2 cytokine levels. Heated raw milk did not show an asthma-protective effect, which indicates the involvement of heat-sensitive components. Besides causal evidence, this study provides the basis for further mechanistic studies.

Formats:

Article       PubReader     ePub (beta)    PDF (3.0M)     Citation

(You must be logged in to add and reply comments)

Interasma on Twitter

Interasma Top story: The Lancet on Twitter: "In 2015, #pollution was responsible for 3 ti… https://t.co/67ta0PuWEX, see more https://t.co/CpGiFuOOYd
32mreplyretweetfavorite
Interasma RT @euforea: Inauguration of the Euforea fund at LUF, paving the way for better research in airway diseases. @LeuvenU https://t.co/D8wOLuKz
6hreplyretweetfavorite
Interasma RT @Aller_MD: Tracing cell death pathway points to drug targets for brain damage, kidney injury, #asthma https://t.co/aUrjIceDa9
6hreplyretweetfavorite
6hreplyretweetfavorite
Interasma Interasma https://t.co/benOb7sQz7 - top stories by @FloodBreda
8hreplyretweetfavorite

Editor: Juan C. Ivancevich, MD

Copyright © Interasma 2003-2017  •  Terms of Use  •  Privacy Policy  •  Contact Us  •  Sitemap

Powered by FREI SA

InterAsma